Spectrometry is the measurement of the interactions between light and matter, and the reactions and measurements of radiation intensity and wavelength. In other words, spectrometry is a method of studying and measuring a specific spectrum.
General Spectrometry
Contact Details
Dr. Ofra Moshel
Faculty of Medicine ,School of Pharmacy Building
Ein Karem Campus
The Hebrew University
Tel: +972-2-6758462
E-mail: oframo@savion.huji.ac.il
The equipment available for use in the Laboratory is listed below:
UV-VIS Spectrophotometer-Ultrospec 2100 pro
In addition to measuring absorbance and concentration, it provides stored routines for nucleic acid quantitation and also a standard curve routine for protein determination. Wavelength scan with zoom, absorbance changes with time, reaction rate determinations, and standard curves can be displayed and printed out. User defined equations can be entered using the multi-wavelength mode and up to 18 methods can be saved.
FTIR-Smart ITR Nicolet iS10
Fourier-transform infrared spectroscopy (FTIR) is a technique used to obtain an infrared spectrum of absorption or emission of a solid, liquid or gas. An FTIR spectrometer simultaneously collects high-spectral-resolution data over a wide spectral range.
Refractometer - B+S Digital RFM970-T
High precision Peltier temperature controlled refractometer
Range – 1.30 to 1.70 RI
Up to 6 decimal places RI
7” High definition touchscreen
Temperature Stability Checks
Fluorometer- Jasco FP-6500
an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample. A certain excitation wavelength is selected, and the emission is observed either at a single wavelength, or a scan is performed to record the intensity versus wavelength, also called an emission spectra
NanoDrop- ND- 1000
a full-spectrum spectrophotometer (UV and visible spectrum, 220-750 nm) for measuring the absorbance of DNA, RNA, proteins and dyes
Polarimeter - B+S Single Wavelength ADP450
uses standard ICUMSA polarimeter tubes with a maximum length of 200mm
contain optics, featuring interference filter. Combined with a photodiode detector it allows the reading of samples of up to 3.0 OD at the commonly used sodium (589nm) wavelength. feature angular and sugar scales (ISS) and can be programmed with user scales as well as industry standard methods for displaying invert sugar, inversion (A-B) or when applying other factors such as tube length and concentration, Specific Rotation (or concentration when entering specific rotation).